Changes in vaginal microbiome after focused ultrasound treatment of high-risk human papillomavirus infection with low-grade cervical lesions

In this study, the changes of vaginal microbiome before and after focused ultrasound (FU) treatment were evaluated to explore the possible mechanism of FU in the treatment of high-risk human papillomavirus (HR-HPV) infection.This study was nested in the FU arm of a prospective cohort study. A total of 74 patients diagnosed with HR-HPV infection with cervical low-grade squamous intraepithelial lesion (LSIL) who met the inclusion criteria were enrolled in this study from October 2020 to November 2021, and these patients were treated with FU. We used 16S ribosomal RNA (16S rRNA) gene amplicon sequencing to profile the vaginal microbiota composition of patients before and 3 months after FU treatment.