Analysis of the effect of PSF on the gene expression profile in neuronal cells

The multi-domain RNA-binding protein (RBP), polypyrimidine tract-binding protein (PTB)-associated splicing factor (PSF) or splicing factor, proline and glutamine-rich (SFPQ), regulate alternative splicing and transcriptional activation/repression by binding to the target RNA molecules. However, the gene regulatory mechanism of PSF in human neuronal cells is still unclear. We investigated the effect of PSF knockdown on the gene expression profile by RNA-seq using human neuronal cells, SH-SY5Y. Overall design: We treated SH-SY5Y cells with siPSF or siControl. After 72 h incubation, RNA-seq analysis was performed.