Characterization of miR-34 effectors in colorectal cancer cells.

MiR-34a and miR-34b/c encoding genes represent direct targets of the p53 tumor suppressor. MiR-34a/b/c mediate tumor suppression by p53 via down-regulation of the expression of specific target mRNAs. Here, we characterized the miR-34 effectors using a CRISPR/Cas9-approach in HCT116 cells with or without 5-FU treatment. Overall design: HCT116 wildtype cells and HCT116 miR-34a/b/c-KO cells were treated with DMSO or 5-FU for 48 hours. For each group, RNAs from three biological replicates were obtained.