KDM6B interacts with TFDP1 to activate P53 signalling in regulating mouse palatogenesis

The goal of this study is to compare transcriptome profiling (RNA-seq) of palatal tissue at E12.5 between control and Kdm6b mutant mice Methods: mRNA profiles of control and Kdm6b mutant mice were generated by deep sequencing, in triplicate, using Illumina Hiseq 4000. The quality of RNA samples was determined using an Agilent 2100 Bioanalyzer and all samples for sequencing had RNA integrity numbers >9.本研究旨在对比对照组与Kdm6b基因突变小鼠胚胎发育第12.5天（E12.5）腭组织的转录组谱分析（RNA-seq）结果。方法：采用Illumina HiSeq 4000测序平台，以三次生物学重复的方式开展深度测序，获取对照组及Kdm6b基因突变小鼠的mRNA表达谱。使用Agilent 2100生物分析仪对RNA样本的质量进行检测，所有用于测序的样本其RNA完整性数（RNA integrity number, RIN）均大于9。