Regulatory mechanism of proanthocyanidins in grape peels by vvi-miR828a and its target gene VvMYBPA1

The present research work was carried out to investigate underlying regulatory mechanism of target genes in grape cultivars Italia and Benitaka. miRNA and transnscriptomic sequencing technology were employed to characterize the profiles of miRNAs and transcripts of grape peels at 10 and 11 weeks post flowering (10 wpf and 11 wpf). The results revealed that the expression level of vvi-miR828a in Italia at 10 wpf and 11 wpf was significantly higher than that in Benitaka. miRNA-seq analysis predicted MYBPA1 as target gene of vvi-miR828a. In transcriptome analysis, the expression level of VvMYBPA1 gene in Benitaka was significantly higher than that in Italia, and the TPM values (expression levels) of VvMYBPA1 and miR828a also showed an evident negative correlation.