Glycan microarray screening analysis of LPyV VP1 pentamers.

Glycan microarray analysis of LPyV VP1 showing selective binding to the short 3SL and 3SLN-related trisaccharide probes with ring-opened flexible cores but not to the N-glycan probe with 3SLN-terminating antennae, complex gangliosides, nor to any of the 6SL and 6SLN-related probes. Numerical scores for the binding intensity are shown as means of fluorescence intensities of duplicate spots at 2 and 5 fmol/spot. Error bars represent half of the difference between the two values. The microarrays consisted of lipid-linked oligosaccharide probes and the sequences are listed in Table S1. The probes are arranged according to terminal sialic acid linkage, oligosaccharide backbone chain length and sequence. The various types of terminal sialic acid linkage are indicated by the colored panels as defined at the bottom of the figure. The expanded region of the microarray highlights the sequences of the probes bound and the selective binding of LPyV VP1 to 3SL and 3SLN-related trisaccharide probes.