Effects of acclimation temperature and feed restriction on the metabolic performance of Green Sturgeon

Green Sturgeon (Acipenser medirostris) are an anadromous threatened species of sturgeon found along the Pacific coast of North America. The southern Distinct Population Segment (sDPS) only spawns in the Sacramento River and is exposed to water temperatures kept artificially cold for the conservation and management of winter-run Chinook Salmon (Oncorhynchus tshawytscha). Past research has demonstrated costs of cold-water rearing including reduced growth rates, condition, and survivorship of juvenile Green Sturgeon. Our research investigates how the multiple stressors of water temperature and food limitation influence the metabolic performance and capacity of Green Sturgeon. We reared Green Sturgeon at two temperatures (13 and 19°C) and two ration amounts (100% and 40% of optimal feed). We then measured the routine (RMR) and maximum (MMR) metabolic rates of sturgeon acclimated to these rearing conditions across a range of acute temperature exposures (11 to 31°C). Among both temperature ac..., The juvenile Green Sturgeon used in this experiment were spawned from wild nDPS adults caught on the Klamath River by fishermen of the Yurok Tribe in April 2016. Adult fish were transported to the Center for Aquatic Biology and Aquaculture (CABA) at UC Davis where they were spawned in accordance with methodology from Van Eenennaam et al. (2001). Eggs were held at 15°C until hatch, afterwards larvae were reared at 18°C in 815 L circular tanks. Larval fish were provided continuous flow of aerated, non-chlorinated fresh water from a dedicated well. After the development of exogenous feeding (ca. 15 days post hatch [dph], 0.1g, 3cm total length [LT]; Van Eenennaam et al. 2001) fish were fed continuously ad libitum with semi-moist pellets (Ragen, Inc.; www.rangen.com) until the experiment began.
This experiment utilized at 2x2 design with fish experiencing one of two acclimation temperatures (13 or 19°C) and one of two feed rates (100% optimal feed rate [OFR] or 40% of optimal feed rate [LFR..., , # Effects of acclimation temperature and feed restriction on the metabolic performance of Green Sturgeon

[https://doi.org/10.25338/B8HS83](https://doi.org/10.25338/B8HS83)

Data provided were gathered from metabolic trials on juvenile Green Sturgeon as described in the Manuscript \"Effects of Acclimation Temperature and Feed Restriction on the Metabolic Performance of Green Sturgeon\" by Zillig et al. (in review). Metabolic trails were conducted on fish acclimated to one of two temperatures (13 or 19C) and provided one of two rations (an Optimal Feed Rate [OFR] or Low Feed Rate [LFR]). Each fish was trialed in a Loligo Swim tunnel and we measured both a Resting Metabolic Rate (RMR) and a Maximum Metabolic Rate (MMR), from which we could then calculate the Aerobic Scope (AS = MMR - RMR) and Factorial Aerobic Scope (FAS = MMR/RMR). We additionally took metrics on fish mass, length, and condition factor.

## Description \"Dryad\_Green\_Sturgeon\_Metabolism\_2023.csv\"

The dataset contains al...