Transcriptome and translatome profiling of warm temperature (35°C -> 38.5°C) treated mouse embryonic fibroblast cells

To see a possible effect of a physiological warm temperature shift (WTS, 35°C to 38.5°C) on Per2 transcription and translation in mammalian cells, we performed RNA-seq and Ribo-seq using dexamethasone-synchronized mouse embryonic fibroblast cells with or without WTS treatment at a circadain rising phase of Per2. We used Dex-synchronized MEF cells treated with or without 2-h WTS. WTS was applied 24 h after Dex synchronization (i.e., circadian rising phase of Per2). Three independent experiments were performed.