Methylome profiling of GADD45 deficient mouse embryonic stem cells

In order to study the physiological impact of GADD45 proteins on DNA methylation, we compared the methylation levels of Gadd45a,b,g triple knockout (TKO) and wild-type (Control) mouse embryonic stem cells (mESC) via whole genome bisulfite sequencing (WGBS). Overall design: Gadd45a,b,g triple knockout (TKO) mouse embryonic stem cells (mESC) were generated using the CRISPR/Cas9 system. Control clones were generated using the same system but without specific gRNA. Genomic DNA was extracted from two Gadd45 TKO and two Control mESC clones and subjected to whole-genome bisulfite sequencing (WGBS).