E-cigarettes induce dysregulation of autophagy leading to endothelial dysfunction in pulmonary arterial hypertension

To investigate whether differential susceptibility exists between healthy and PAH patients to e-cig exposure and the molecular mechanisms, human iPSC lines from two healthy volunteers and two FPAH patients were first differentiated into ECs. iPSC-ECs were then treated with and without e-cigarette extract for 24 hours. RNA-seq of iPSC-ECs with and without e-cigarette extract treatment over a period of 24 hours.