RNASeq of mouse, human, and non-human primate primary dermal fibroblasts to poly(I:C) transfection

Here, we performed RNA-Seq on mRNA isolated from primary dermal fibroblasts of human, mice and multiple non-human primate species transfected with polyinosinic-polycytidylic acid (polyI:C), a synthetic dsRNA analog that invokes a strong interferon-mediated response, or mock transfected. For each of the ten species, primary fibroblasts were acquired from three donors. Cells from each donor were transfected in triplicate with 0.5 ug poly(I:C)/well in a six well plate (~0.05 ug/cm2) or mock-transfected (all transfection mixture components minus poly(I:C)) in triplicate. Thus, there were a total of 180 samples (10 species x 3 donors x 6 samples per donor).