Bos taurus Transcriptome or Gene expression

Bovine embryonic stem cells (bESCs) culturing is performed based on information obtained from culturing of the mouse and human inner cell mass (ICM) because of a lack of reliable transcriptional information on the ICM and trophectoderm (TE) lineages in cattle. This limitation has greatly affected bESCs research progress. Here, we separated a high-purity (>90%) ICM and TE from bovine blastocysts by magnetic-activated cell sorting and analyzed their transcriptomes by single-cell RNA-seq.