Rhizosphere metagenomic sequencing and Illumina MiSeq sequencing

Growth substrates containing each soil type were collected at the initial time point into 2 mL sterilized microcentrifuge tubes, serving as 'Bulk' samples. These included two treatments: BMO: sterile artificial soil mixed with OE soil inoculum and BMR: sterile artificial soil mixed with RNAi soil inoculum. Rhizosphere samples collected before transplanting and pathogen inoculation were designated as 'MRT0', comprising: MO: rhizosphere derived from OE soil microbiome and MR: rhizosphere derived from RNAi soil microbiome. Fourteen days post-pathogen inoculation, samples were classified as 'MRT14' with four treatments: HMO: OE rhizosphere without Fpmd MR5 inoculation, FMO: OE rhizosphere with Fpmd MR5 inoculation, HMR: RNAi rhizosphere without Fpmd MR5 inoculation, and FMR: RNAi rhizosphere with Fpmd MR5 inoculation.