Escherichia coli K-12 strain:k12 | isolate:34154 Raw sequence reads

Quinolones are broad-spectrum antibacterial agents that target bacterial topoisomerases (DNA gyrase and topoisomerase IV) and, as a consequence, inhibit DNA replication and transcription. Quinolones are commonly used for treatment of infections both in human and veterinary medicine, in particular against ESBL pathogen E. coli. However due to the potential for undesirable release of shigatoxin (Stx) by dying and dead bacterial cells, quinolones are usually avoided against Enterohemorrhagic E. coli (EHEC). Subinhitory concentrations (SICs) of quinolones identified in the environmental conditions have an important role in the evolution and the dissemination of antibiotic resistance, eg. among ESBL E. coli and/or EHEC. SIC of quinolones has also a significant effect on Stx realise via bacteriophage induction. Only few studies have reported the effect of SICs of quinolones on pathogenic E. coli while no global transcriptomic approaches have been conduction on STEC-ESBL. The global impat of quinolones on pathogenic and ESBL E. coli need to be clarified. In order to do this, this tudy evaluated the impact of SIC of ciprofloxacin, most commonly used fluroquinolone, on transcriptome of 3 E. coli isolates: the reference E. coli K12 (#34154), one EHEC (#30934) and one EHEC-ESBL (#22207) from bovin origin.