Vitamin D receptor ChIP-Seq in primary prostate epithelial cells

We investigated the genomic activity of vitamin D in primary prostate epithelial cells (PrE) through vitamin D receptor (VDR)-ChIP-sequencing. PrE cells treated with 50 nM 1,25D showed nuclear localization the VDR within 1 hr, persisting until 4 hr, and a 2 hr treatment was selected for ChIP-seq. Rigorous testing of the ChIP conditions and VDR antibody were performed. Nearly 5,000 VDR binding sites were identified. Overall design: One primary prostate epithelial cell line treated with ethanol (control) or 50 nM 1,25-VitaminD3 for 2 hr