Immunological memory diversity in the human upper airway

Limited CITE-seq antibody panel and 5' gene expression profiling of human nasopharyngeal cells collected with flocked swabs, paired with TCR and BCR sequencing of NP lymphocytes. FACS-sorted SARS-CoV-2 antigen-specific memory B and T cells with 5' gene expression profiling and scBCR and scTCRseq. Overall design: 10x Genomics 5' immune profiling using BioLegend TotalSeq-C TBNK, hashtag antibodies, and SARS-CoV-2 labeled probes.