Proteomic analysis of the cerebral cortex of 8-month-old male Bphl-/- and Bphl+/+ mice

[ENG:]This file contains data regarding comparative proteomics of the cerebral cortex of 8-month-old male Bphl-/- (n=6) and Bphl+/+ (n=5) mice housed under standard conditions. Cortex protein isolation was performed at the Department of Biochemistry and Biotechnology, Poznań University of Life Sciences, using TAEB buffer with 0.1% SDS. Disulfide bridges were then reduced using TCEP and blocked with MMTS. Proteins were digested with trypsin. Peptides were labeled with TMTpro 16-plex and fractionated in a reversed-phase system. Proteomic analysis was performed using an Evosep One coupled to an Orbitrap Exploris 480 mass spectrometer at the Mass Spectrometry Laboratory at the Institute of Biochemistry and Biophysics of the Polish Academy of Sciences in Warsaw. Samples from Bphl-/- mice are marked with tags 1, 3, 5, 7, 11, and 13, while those from Bphl+/+ mice are marked with tags 4, 6, 8, 10, and 12.The CSV file contains data in the following columns: Protein ID, Gene names, Fasta headers, id, Student's T-test Significant BPHL_WT, Student's T-test q-value BPHL_WT, Student's T-test Difference BPHL_WT, Student's T-test Fold change BPHL_WT, Mol. weight [kDa], Q-value, MS/MS count, Razor + unique peptides TMT16plex, Unique peptides TMT16plex, Sequence coverage TMT16plex [%], TMT16plex signals.