This is a cDNA library constructed from multiple starved adults pooled prior to RNA extraction, using an Illumina TruSeq RNA v2 protocol, and sequenced in multiplex 2x100 bp on an Illumina HiSeq 2000.
Background: There are challenges in generating mRNA-Seq data from whole-blood derived RNA as globin gene and rRNA are frequent contaminants. Given the abundance of erythrocytes in whole blood, globi