Saccharomyces cerevisiae strain:delta|(-2)|-7B-YUNI300 Genome sequencing

The present study was designed to answer three questions. First, do ribonucleotides incorporated during nuclear DNA replication in RER-defective yeast strains elevate the rate of loss-of-heterozygosity (LOH), and non-allelic homologous recombination (NAHR) leading to chromosomal translocations and copy number alterations? If so, do elevated LOH or NAHR rates depend on ribonucleotide incorporated by Pol a, Pol d or Pol e? Thirdly, do elevated LOH or NAHR rates depend on Top1? We show by whole genome sequence analysis that loss of RNase H2 activity increases loss-of-heterozygosity (LOH) in Saccharomyces cerevisiae diploid strains harboring a mutant allele of Pol e.