RNAi of Thy-ncR1 ncRNA in HPB-ALL cells (CBX142). unidentified

In our recent research, we identified several mRNA-like non-coding RNA express in the thymus. Expression profiling of these thymus-specific ncRNAs in 17 T-cell leukemia cell lines derived from various stages of T-cell maturation revealed that HIT14168 ncRNA, named Thy-ncR1, was specifically expressed in the cell lines derived from immature T-cells. To examine the biological function of Thy-ncR1, each isoform was selectively degraded using RNAi in HPB-ALL cells. The siRNA 1, which corresponds to a sequence in exon 1, depleted all the isoforms, and siRNA 3-1 and 3-2 depleted two major isoforms (Ex1-2-3 and Ex1-3). Microarray analysis were used to identify genes whose expression was controlled by Thy-ncR1 .