In this document the retention times, calculated retention indices and calculated delta retention indices can be found that were used for the thesis on Exploring the Effects of LC Parameters on Retent
(A) SDS-PAGE and Coomassie blue staining of the whole cell lysate corresponding to E. coli BL21 transformed with pCold-TF-AcLAP plasmid (lane 1), soluble fraction of the sonicated cell lysate (lane 2)
A. Protein topology showing the presence of signal peptide and/or transmembrane helices in the protein signal peptide spans from 1–34 amino acids, whereas transmembrane domain spans from 7–29 amino ac