Colonic contractility recordings following spinal cord injury in mice with dietary fiber interventions

Here, we performed colonic contractility measurements in mice following spinal cord injury, with or without a dietary fiber intervention at 2 weeks post-injury. Provided here are is a .zip file containing the raw ABF measurement files necessary for analysis of the frequency and amplitude of contractions. Breifly, data were acquired using AxoClamp 900A (Axon Instruments). Each tissue segment was recorded for at least 30min in 5min gap-free files. 15min of stable recordings were selected from the middle of each recording session for analysis. Using Clampfit software (Molecular Devices, RRID:SCR_011323), data files were filtered at 300Hz (Bessel 8-pole) and reduced by a factor of 100. Files were concatenated and the baseline was adjusted based on overall slope. Amplitude was calculated by subtracting the minimum value of the whole trace from the value of the peak being assessed.