Single-cell sequencing screening of biomarkers associated with Lipophagy in the MPTP model mice of Parkinson's disease

MPTP model mice brains (PD, n=3; CON, n=3) underwent scRNA-seq. Post-QC, cells were clustered & annotated. Differential infiltrated cells (DICs) identified between PD & CON. DICs further sub-clustered. DIC-DEGs & DE-LRGs determined. Overlapping DIC-DEGs & DE-LRGs yielded candidate genes. CytoNCA's 5 algorithms screened biomarkers. Biomarker expression compared between PD & CON. Cell-cell communication & pseudotime analyses explored communication & differentiation.