Supplemental data sets for Raudabaugh et al., Where are they hiding? Testing the body snatchers hypothesis in pyrophilous fungi

The data set contains Supplemental data sets for the Manuscript entitled "Where are they hiding? Testing the body snatchers hypothesis in pyrophilous fungi." Environmental sampling: Amplification of nuclear DNA regions (ITS1 and ITS2) were completed using the Fluidigm Access Array and the resulting amplicons were sequenced on an Illumina MiSeq v2 platform runs using rapid 2 × 250 nt paired-end reads. Illumina sequencing run amplicons that were size selected into <500nt and >500nt sub-pools, then remixed together <500nt: >500nt by nM concentration in a 1x:3x proportion. All amplification and sequencing steps were performed at the Roy J. Carver Biotechnology Center at the University of Illinois Urbana-Champaign. ITS1 region primers consisted of ITS1F (5'-CTTGGTCATTTAGAGGAAGTAA-'3) and ITS2 (5'-GCTGCGTTCTTCATCGATGC-'3). ITS2 region primers consisted of fITS7 (5'-GTGARTCATCGAATCTTTG-'3) and ITS4 (5'-TCCTCCGCTTATTGATATGC-'3). Supplemental files 1 through 5 contain the raw data files. Supplemental 1 is the ITS1 Illumina MiSeq forward reads and Supplemental 2 is the corresponding index files. Supplemental 3 is the ITS2 Illumina MiSeq forward reads and Supplemental 4 is the corresponding index files. Supplemental 5 is the map file needed to process the forward reads and index files in QIIME. Supplemental 6 and 7 contain the resulting QIIME 1.9.1. OTU tables along with UNITE, NCBI, and CONSTAX taxonomic assignments in addition to the representative OTU sequence. Numeric samples within the OTU tables correspond to the following: 1 Brachythecium sp. 2 Usnea cornuta 3 Dicranum sp. 4 Leucodon julaceus 5 Lobaria quercizans 6 Rhizomnium sp. 7 Dicranum sp. 8 Thuidium delicatulum 9 Myelochroa aurulenta 10 Atrichum angustatum 11 Dicranum sp. 12 Hypnum sp. 13 Atrichum angustatum 14 Hypnum sp. 15 Thuidium delicatulum 16 Leucobryum sp. 17 Polytrichum commune 18 Atrichum angustatum 19 Atrichum angustatum 20 Atrichum crispulum 21 Bryaceae 22 Leucobryum sp. 23 Conocephalum conicum 24 Climacium americanum 25 Atrichum angustatum 26 Huperzia serrata 27 Polytrichum commune 28 Diphasiastrum sp. 29 Anomodon attenuatus 30 Bryoandersonia sp. 31 Polytrichum commune 32 Thuidium delicatulum 33 Brachythecium sp. 34 Leucobryum glaucum 35 Bryoandersonia sp. 36 Anomodon attenuatus 37 Pohlia sp. 38 Cinclidium sp. 39 Hylocomium splendens 40 Polytrichum commune 41 negative control 42 Soil 43 Soil 44 Soil 45 Soil 46 Soil 47 Soil If a sample number is not present within the OTU table; either no sequences were obtained or no sequences passed the quality filtering step in QIIME. Supplemental 8 contains the Summary of unique species per location.

该数据集收录了论文《他们藏身何处？检验嗜热真菌中的‘尸体采集者’假说》的补充数据集。环境样本采集过程中，采用Fluidigm Access Array对核DNA区域（ITS1和ITS2）进行扩增，随后在Illumina MiSeq v2平台进行测序，使用快速2 × 250碱基配对端读取。Illumina测序得到的扩增子根据大小被筛选进入<500nt和>500nt的子池，然后按照1x:3x的比例，通过nM浓度重新混合<500nt与>500nt。所有扩增和测序步骤均在伊利诺伊大学厄巴纳-香槟分校的Roy J. Carver生物技术中心完成。ITS1区域引物包括ITS1F（5'-CTTGGTCATTTAGAGGAAGTAA-'3）和ITS2（5'-GCTGCGTTCTTCATCGATGC-'3）。ITS2区域引物包括fITS7（5'-GTGARTCATCGAATCTTTG-'3）和ITS4（5'-TCCTCCGCTTATTGATATGC-'3）。补充文件1至5包含原始数据文件。补充1为ITS1 Illumina MiSeq正向读取，补充2为相应的索引文件。补充3为ITS2 Illumina MiSeq正向读取，补充4为相应的索引文件。补充5为QIIME处理正向读取和索引文件所需的映射文件。补充6和7包含QIIME 1.9.1版本的OTU表，以及UNITE、NCBI和CONSTAX的分类学分配，还包括代表性OTU序列。OTU表中的数字样本对应以下物种：1. Brachythecium sp. 2. Usnea cornuta 3. Dicranum sp. 4. Leucodon julaceus 5. Lobaria quercizans 6. Rhizomnium sp. 7. Dicranum sp. 8. Thuidium delicatulum 9. Myelochroa aurulenta 10. Atrichum angustatum 11. Dicranum sp. 12. Hypnum sp. 13. Atrichum angustatum 14. Hypnum sp. 15. Thuidium delicatulum 16. Leucobryum sp. 17. Polytrichum commune 18. Atrichum angustatum 19. Atrichum angustatum 20. Atrichum crispulum 21. Bryaceae 22. Leucobryum sp. 23. Conocephalum conicum 24. Climacium americanum 25. Atrichum angustatum 26. Huperzia serrata 27. Polytrichum commune 28. Diphasiastrum sp. 29. Anomodon attenuatus 30. Bryoandersonia sp. 31. Polytrichum commune 32. Thuidium delicatulum 33. Brachythecium sp. 34. Leucobryum glaucum 35. Bryoandersonia sp. 36. Anomodon attenuatus 37. Pohlia sp. 38. Cinclidium sp. 39. Hylocomium splendens 40. Polytrichum commune 41. 负对照组 42. 土壤 43. 土壤 44. 土壤 45. 土壤 46. 土壤 47. 土壤。若OTU表中无样本编号，则表示未获得序列或序列未通过QIIME的质量过滤步骤。补充8包含每个地点独特物种的汇总。