Deep sequencing results for htFuncLib libraries of GFP
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Deep sequencing results for unsorted and sorted samples of active site mutations focused libraries of GFP. The file contains fastq files separated by samples. Each sample has pair-end reads. Samples are: 10_n_x_any_GFP: nohbonds library sorted for GFP fluorescence. 14_w_x_GFP_GFP: hbonds library sorted for GFP fluorescence. 18_w_x_any_any: ignore. 21_n_old_GFP_Cy: ignore. 25_w_O_unsorted: hbonds library, unsorted. 4_n_O_Cyan_Cyan: nohbonds library sorted for AmCyan fluorescence. 8_n_x_Cyan_GFP: nohbonds library sorted for GFP fluorescence. 11_n_x_any_any: ignore. 15_w_x_Cyan_GFP: hbonds library sorted for GFP fluorescence. 19_n_old_GFP: ignore. 22_n_old_Cyan: ignore. 26_w_X_unsorted: hbonds library, unsorted. 5_n_O_any_any: ignore. 9_n_x_Cyan_Cyan: nohbonds library sorted for AmCyan fluorescence. 12_w_O_GFP_GFP: hbonds library sorted for GFP fluorescence. 16_w_1_CyanCyan: hbonds library sorted for AmCyan fluorescence. 1_n_O_GFP_GFP: nohbonds library sorted for GFP fluorescence. 23_n_O_unsorted: nohbonds library, unsorted. 2_n_O_GFP_Cyan: nohbonds library sorted for AmCyan fluorescence. 6_n_x_GFP_GFP: nohbonds library sorted for GFP fluorescence. 13_w_O_CyanCyan: hbonds library sorted for AmCyan fluorescence. 17_w_x_any_GFP: hbonds library sorted for GFP fluorescence. 20_n_old_GFPCy: ignore. 24_n_X_unsorted: nohobnds library, unsorted. 3_n_O_Cyan_GFP: nohbonds library sorted for GFP fluorescence. 7_n_x_GFP_Cyan: nohbonds library sorted for AmCyan fluorescence. <br> Please cite the reference bioXriv paper or any publisehd paper with the same title...
本数据集为绿色荧光蛋白(Green Fluorescent Protein, GFP)活性位点突变聚焦文库的未分选与分选样本的深度测序结果。文件包含按样本分组的FASTQ格式测序文件,每个样本均带有双端测序读段。各样本详情如下:
10_n_x_any_GFP:无氢键(nohbonds)文库,经GFP荧光分选
14_w_x_GFP_GFP:有氢键(hbonds)文库,经GFP荧光分选
18_w_x_any_any:忽略不计
21_n_old_GFP_Cy:忽略不计
25_w_O_unsorted:有氢键(hbonds)文库,未分选
4_n_O_Cyan_Cyan:无氢键(nohbonds)文库,经青色荧光蛋白(AmCyan)荧光分选
8_n_x_Cyan_GFP:无氢键(nohbonds)文库,经GFP荧光分选
11_n_x_any_any:忽略不计
15_w_x_Cyan_GFP:有氢键(hbonds)文库,经GFP荧光分选
19_n_old_GFP:忽略不计
22_n_old_Cyan:忽略不计
26_w_X_unsorted:有氢键(hbonds)文库,未分选
5_n_O_any_any:忽略不计
9_n_x_Cyan_Cyan:无氢键(nohbonds)文库,经AmCyan荧光分选
12_w_O_GFP_GFP:有氢键(hbonds)文库,经GFP荧光分选
16_w_1_CyanCyan:有氢键(hbonds)文库,经AmCyan荧光分选
1_n_O_GFP_GFP:无氢键(nohbonds)文库,经GFP荧光分选
23_n_O_unsorted:无氢键(nohbonds)文库,未分选
2_n_O_GFP_Cyan:无氢键(nohbonds)文库,经AmCyan荧光分选
6_n_x_GFP_GFP:无氢键(nohbonds)文库,经GFP荧光分选
13_w_O_CyanCyan:有氢键(hbonds)文库,经AmCyan荧光分选
17_w_x_any_GFP:有氢键(hbonds)文库,经GFP荧光分选
20_n_old_GFPCy:忽略不计
24_n_X_unsorted:无氢键(nohbonds)文库,未分选(原文笔误为“nohobnds”,已修正为标准术语)
3_n_O_Cyan_GFP:无氢键(nohbonds)文库,经GFP荧光分选
7_n_x_GFP_Cyan:无氢键(nohbonds)文库,经AmCyan荧光分选
请引用该BioRxiv预印本论文或同标题已发表论文进行标注。
提供机构:
figshare
创建时间:
2023-01-19
搜集汇总
数据集介绍

背景与挑战
背景概述
该数据集包含GFP活性位点突变库的深度测序结果,分为有氢键和无氢键库,样本经过不同荧光类型(GFP或AmCyan)的排序或未排序处理。数据集提供了配对的fastq文件,适用于蛋白质突变研究和生物信息学分析。
以上内容由遇见数据集搜集并总结生成



