Intracellular pH measurements in the Pacific oyster (Crassostrea gigas)

Intracellular pH was measured using the dye BCECF (live cell imaging) in the mantle epithelial cells of the Pacific oyster (C. gigas). Intracellular pH was measured under the presence of modified seawater solutions (low sodium, low bicarbonate) and specific inhibitors for acid-base proteins were used. Value are presented as means.