Effect of endothelial specific inhibition of Brg1 on histone marker H3K4me3 in the zebrafish genome [ChIP-seq]

To decipher the effect of endothelial specific inhibition of Brg1 on histone marker H3K4me3 in the zebrafish genome during zebrafish heart regeneration, we achieved endothelium-specific over-expression of DN-xBrg1 by using the compound zebrafish line consisting of Tg(ubi:loxp-DsRed-STOP-loxp-DN-xBrg1; kdrl:CreER) (defined as DN), while we used Tg(ubi:loxp-DsRed-STOP-loxp-DN-xBrg1) as control (Ctrl) in the presence of 4-HT starting at 3 days before ventricular resection. The whole ventricles from CtrlK and DNK hearts at 7 dpa were subjected to ChIP-seq analysis using H3K4me3 antibody. Overall design: Chromatin immunoprecipitation DNA-sequencing (ChIP-seq) for H3K4me3 in Ctrl and DN amputated ventricles at 7dpa.