Abrogation of WNT signaling by dominant-negative TCF4. Homo sapiens

To assess cellular changes upon abrogation of the WNT-signaling pathway, we induced expression of a dominant-negative T-cell factor 4 (TCF4). This showed a remarkable overlap with activation of the unfolded protein response (UTR) in the same colon cancer cell line. Overall design: 500,000 cells were plated in a 10 cm2 plate, and the following day, treated for 20 hrs with doxycycline (Dox) or vehicle. Three independent biological replicates per treatment.