Integrated transcriptomic and proteomic analysis reveals inflammatory activation and blood-brain barrier disruption during meningitis-associated Escherichia coli infection

Figure S1. Differential expression analysis and principal component analysis of transcriptomic and proteomic data. (A) Volcano plot of transcriptomic data showing differential gene expression in RS218 compared to mock conditions. Differentially expressed genes are shown in red (upregulated) and blue (downregulated). (B) Principal component analysis (PCA) of transcriptomic data based on differentially expressed genes. (C) Volcano plot of proteomic data showing differential protein expression in RS218 compared to mock conditions. Differentially expressed proteins are shown in red (upregulated) and blue (downregulated). (D) Principal component analysis (PCA) of proteomic data based on all differentially expressed proteins.Figure S2. Blood biochemical and hematological parameters in mice following RS218 infection. (A) Blood biochemical parameters measured in mock-infected controls and in mice at 4 and 12 hours post-infection. Parameters include albumin/globulin ratio, total bilirubin, amylase, creatine kinase, triglyceride, and AST/ALT ratio. (B) Hematological parameters including mid-sized cell count (MID), mid-sized cell percentage (MID%), red blood cell count (RBC), hemoglobin (HGB), hematocrit (HCT), mean corpuscular volume (MCV), mean corpuscular hemoglobin (MCH), mean corpuscular hemoglobin concentration (MCHC), red cell distribution width coefficient of variation (RDW_CV), red cell distribution width standard deviation (RDW_SD), mean platelet volume (MPV), platelet distribution width (PDW), platelet large cell ratio (P_LCR), platelet large cell count (P_LCC), and plateletcrit (PCT).Table S1. Differentially expressed genes in transcriptomics.Table S2. Differentially expressed proteins in proteomics.Table S3. Abundance of genes co-upregulated in transcriptome and proteome.Table S4. Top 100 enriched terms from comparative analysis of upregulated transcriptome and proteome.Table S5. Abundance of genes co-downregulated in transcriptome and proteome.Table S6. Top 100 enriched terms from comparative analysis of downregulated transcriptome and proteome.Table S7. Shared enriched terms between upregulated and downregulated transcriptome and proteome.Table S8. Transcriptome and proteome discordant genes.