Transcriptome analyses of Colletotrichum orbiculare during appressorium formation in planta

To elucidate the involvement of MOR (morphogenesis-related NDR kinase network) pathway in regulation of genes that are specifically expressed during appressorium development in planta, transcriptome analyses at the stage of appressorium formation (4 hours post inoculation) and in planta were performed and revealed that MOR is responsible for the expression of a subset of the plant signal induced genes with potential roles in pathogenicity. We collected samples from germinating conidia incubated on cucumber cotyledons (in planta) for 4 h of the MTF4-OX or mtf4Δ strains. Four replicates were performed for each experiment.