Figure 2B Broad neutralization of HCMV strains

<b>Figure 2.</b> <b>Monoclonal antibodies are broadly neutralizing.</b> (a) Increasing concentrations of monoclonal antibodies (ug/ml) were incubated with AD169R (MOI 0.2) to assess neutralizing capacity across epithelial cells, placental-derived trophoblasts and fibroblasts. Antibodies were diluted from 50 mg/mL using 5-fold dilutions for epithelial and trophoblasts cells. The mAbs were diluted using 10-fold dilutions from 100 ug/mL using the same AD169R (MOI 0.2) conditions in fibroblast neutralization assays. Percent infection was quantified using GFP expression at 18 hpi and normalized based on infection with virus only (no antibody). (b) mAb panel was used to neutralize diverse HCMV strains (MOI 0.2) in MRC5 fibroblast cells using a 5-fold serial dilution starting at 50 ug/mL. Relative percent infection at 18 hpi was determined following fixation and indirect immunofluorescence (IF) staining for IE1. All conditions were performed in technical triplicate (n=3) and at minimum using n=2 independent experiments. Error bars represent standard deviation from the mean.