RNA-seq of shE4F1 or shCTRL SUM159 triple negative breast cancer cell line treated or not with Gemcitabine

E4F1 is a ubiquitously expressed zinc-finger protein of the Gli-Kruppel family that was first identified, more than 30 years ago, as a cellular target of the adenoviral oncoprotein E1A13S (Ad type V), required for transcriptional regulation of adenoviral genes. In this study we investigated the impact of direct translational target genes on DNA damage response induced by Gemcitabine. To do so, we performed RNAseq SUM159 triple negative breast cancer cell line. SUM159 was tranfected either with shE4F1 OR shscramble treated or not with Gemcitabine for 24 hours. Overall design: mRNA profiles of SUM159 treated or not with Gemcitabine and transfected either with scramble shRNA or shRNA-E4F1 were generated using TruSeq stranded RNA Library Prep Kit (Illumina) followed by sequencing with 50 bp single-end reads on HiSeq 2500.