Genome-wide binding site profiling of NdgR in Streptomyces coelicolor. Streptomyces coelicolor
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https://www.ncbi.nlm.nih.gov/bioproject/PRJNA254102
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资源简介：
We identified genome-wide binding regions of NdgR in Streptomyces coelicolor using chromatin immunoprecipitation sequencing (ChIP-seq). We constructed 6×myc-tagged NdgR strain using homologous recombination with myc-tagging vector. Analysis of the sequencing data aligned to Streptomyces coelicolor genome database (NC_003888). Overall design: 6x-myc tagged Streptomyces coelicolor M145 was cultured on solid minimal media supplemented with N-acetylglucosamine and asparagine during 36hrs. Anti-myc antibody (9E10) was used for immunoprecipitation (IP). IP-DNA was sequenced using Illumina Genome Analyzer Ⅱx
创建时间：
2014-07-02
