Figure 2

Fig. 2 Generation of lipid radicals and oxidized lipids in PDT-exposed Intralipid. (a) Photodynamic generation of lipid radicals in 1 µM BPD solutions containing increasing concentrations of Intralipid under light irradiation. (b) LC-MS analysis of Intralipid showing summed peak areas of unoxidized (Unox) versus oxidized (Ox) lipids, where oxidized lipids contain -OH and -OOH groups. (c) Summed peak areas of the five most abundant oxidized lipid classes present in Intralipid. (d) Volcano plot displaying individual oxTG, oxPC, and oxPE species with absolute log2 fold change > 1 and -log10 p-value < 0.05. (e) Heatmaps of Z-scores for individual oxTG, oxPC, and oxPE species, arranged in order of the increasing carbon chain length (gray arrow) and the total number of double bonds (unsaturation, side bar).