Processed single-cell RNA-seq data for "Evolutionarily conserved transcriptional regulators control monoaminergic neuron development"

Contents Folder Description 1_Replicate_Processed_Objects Processed per-replicate scRNA-seq objects (post-QC, normalized, annotated). 2_Timepoint_Processed_Objects Integrated, timepoint-specific datasets spanning 0–22 h after egg laying (AEL). 3_FullDataset_Processed_Objects Full integrated dataset across all developmental stages with annotated cell identities. 4_MatureNeuronalDataset_Processed_Objects Subset of mature neuronal populations used for trajectory analyses. 5_MatureNeuronalDataset_Velocity_Objects scVelo-formatted Loom files for RNA velocity analysis. 6_MatureNeuronalDataset_CellRank_Objects Processed CellRank outputs with fate probabilities and latent-time assignments. --- 🧩 Usage Notes These processed objects can be loaded directly into: R (Seurat ≥ v5.2) Python (Scanpy / scVelo ≥ v0.3) Refer to the Methods section of the manuscript for detailed data-processing and analysis pipelines. For reproducibility, all analysis scripts are available at: 🔗 https://github.com/cliftonlewis/2025_Drosophila_scRNAseq_EmbryoNeurogenesis_Monoamine/ --- Keywords Drosophila • single-cell RNA-seq • embryonic neurogenesis • RNA velocity • CellRank • trajectory analysis • monoaminergic neurons • transcription factors • evolution --- ⚖️ License This repository and dataset are made available under the **MIT License**: Copyright (c) 2025 Clifton Lewis and contributors --- Citation If you use this dataset, please cite: > Lewis, C., et al. (2025). Evolutionarily conserved transcriptional regulators control monoaminergic neuron development. [Manuscript in preparation / published in *Journal TBD*]. ---