GNPS - LBNL_Phage_Nitrogen_Novak_Northen_2024

The dataset contains LC-MS files for two experiments:
1. 15N-necromass Isotope Labeling Experiment: 20220124_AG_VN_WFNSFVIC_PhageN
This experiment examines how phage-lysed (gh-1 phage) and uninfected 15N-labeled and unlabeled Pseudomonas putida necromass affects nitrogen uptake by Brachypodium distachyon and the composition of rhizosphere exometabolites.

2. Necromass Incubation Experiment with and without Plants: 20240229_EB_VN_107432-001_LysTxCtrl
This experiment investigates the turnover of bacterial necromass with and without Brachypodium distachyon.

For both experiments, filtered P. putida necromass was supplied to plants growing in EcoFAB 2.0 in sterile conditions for one week. The composition of the necromass medium was analyzed at pre-incubation (t0) and post-incubation (t1) using polar (HILIC) metabolite analysis. Each treatment had n=5 biological replicates.

Work included in publication:

Novak, V., van Winden, M.C.M., Harwood, T.V., Neurath, R., Kosina, S.M., Louie, K.B., Sullivan, M.B., Roux, S., Zengler, K., Mutalik, V.K. and Northen, T.R. (2025), Virocell Necromass Provides Limited Plant Nitrogen and Elicits Rhizosphere Metabolites That Affect Phage Dynamics. Plant, Cell & Environment. https://doi.org/10.1111/pce.15456