Transcriptomic responses of the kidney to development of and recovery from acute decompensated heart failure (ADHF)

We report changes in kidney gene expression in response to development of, and recovery from, ADHF using an ovine model of rapid cardiac pacing (220 beats per minute). Overall design: We obtained kidney biopsies prior to cardiac pacing (day 0), after development of ADHF (day 14) and at the end of the 25-day (non-pacing) recovery period from 6 Coopworth ewes (5 x Baseline, 6 x HF and 5 x Recovery serial samples). To maximise statistical power, the serial samples were supplemented with kidney samples collected post-mortem from animals sacrificed before, during or after the same pacing protocol. This provided a total of 14 x Baseline, 13 x Heart Failure and 8 x Recovery samples for RNA-Seq analysis.