Effects of RA on endoderm marker gene expression.

Stage indicates the stage of treatment; Bead graft, grafting of RA-soaked beads; Culture med, RA was applied to the culture medium; ND, not done;Phenotypes: Cyp26A1, concentration-dependent induction around the bead; HoxB4 and HoxA2, anterior expansion of the domain & increased levels of expression; Hex, comprise embryos collected at HH 5�C6 and HH 14; at HH 5�C6, Hex is partially repressed and at HH 14, Hex is inhibited in thyroid domain, liver domain remains unaffected; Nkx2.1, inhibited thyroid expression; Prox1, repression close to the bead. ��-fibrinogen, Pdx1 and CdxA, no effect.