Eugenia Morselli, Guillermo Mariño, Martin v. Bennetzen, Tobias Eisenberg, Evgenia Megalou, Sabrina Schroeder, Sandra Cabrera, Paule Bénit, Pierre Rustin, Alfredo Criollo, Oliver Kepp, Lorenzo Galluzzi, Shensi Shen, Shoaib Ahmad Malik, Maria Chiara Maiuri, Yoshiyuki Horio, Carlos López-Otín, Jens S. Andersen, Nektarios Tavernarakis, Frank Madeo, Guido Kroemer (2011) CIL:13914, Homo sapiens, permanent cell line cell. CIL. Dataset

Image indicative of 24 h autophagic activity of human colon carcinoma HCT 116 cell co-transfected with a RFP-LC3-encoding plasmid and a SIRT1 variant with a mutation in the nuclear localization signal fused to GFP (mtNLS GFTP-SIRT1) which is virtually restricted to the cytoplasm. Confocal fluorescent images were captured using a confocal fluorescence microscope (TCS SP2; Leica) fitted with an Apochromat 63× 1.3 NA immersion objective. Images were acquired with a camera (DFC 350 FX 1.8.0; Leica) using LAS AF software (Leica) and processed with Photoshop (CS2; Adobe) software. Specifically, picture processing involved cropping of representative areas and linear adjustments of contrast and brightness and was performed using Photoshop (with equal adjustment parameters for all pictures); no explicit γ correction was used. Image: Figure 8C, bottom panel, in Morselli et al. J Cell Biol 192: 615-629

本数据集展示了人结肠癌细胞HCT 116经RFP-LC3编码质粒与核定位信号突变融合至GFP（mtNLS GFTP-SIRT1）的SIRT1变异体共转染后的24小时自噬活性图像。该变异体在细胞质中几乎完全限定。采用共聚焦荧光显微镜（TCS SP2；莱卡）配备阿贝63× 1.3 NA浸没物镜，捕获了共聚焦荧光图像。图像采集使用相机（DFC 350 FX 1.8.0；莱卡）以及LAS AF软件（莱卡）完成，并利用Photoshop（CS2；Adobe）软件进行后期处理。具体而言，图像处理涉及代表性区域的裁剪以及对比度和亮度的线性调整，处理过程中采用Photoshop（对所有图片使用相同的调整参数）；未使用显式γ校正。图像信息见Morselli等人在《细胞生物学杂志》（J Cell Biol）192卷第615-629页的图8C底部。