METHOD FOR DESIGNING RNA BINDING PROTEIN UITILIZING PPR MOTIF， AND USE THEREOF

A method for designing a protein capable of binding in an RNA base selective manner or RNA base sequence specific manner is provided. The protein of the present invention is a protein containing one or more of PPR motifs (preferably 2 to 14 PPR motifs) each consisting of a polypeptide of 30- to 38-amino acid length represented by the formula 1 (wherein Helix A is a moiety of 12-amino acid length capable of forming an α-helix structure， and is represented by the formula 2， wherein， in the formula 2， A 1 to A12 independently represent an amino acid; X does not exist， or is a moiety of 1- to 9-amino acid length; Helix B is a moiety of 11- to 13-amino acid length capable of forming an α-helix structure; and L is a moiety of 2- to 7-amino acid length represented by the formula 3， wherein， in the formula 3， the amino acids are numbered "i" (-1)， "ii" (-2)， and so on from the C-terminus side， provided that Liii to Lvii may not exist)， and combination of three amino acids A1， A4 and Lii， or combination of two amino acids A4， and Lii is a combination corresponding to a target RNA base or base sequence.