NUP98-HBO1-induced gene signature in human CB CD34+ cells

Chronic myelomonocytic leukemia (CMML) is an incurable hematopoietic stem cell malignancy. We identified a novel NUP98-HBO1 fusion from a patient with CMML. HBO1, a histone acetyltransferase (HAT) which belongs to the MYST family, is the first NUP98 fusion partner encodes HAT. To determine the effect of the NUP98-HBO1 fusion on downstream target gene regulation, we performed gene expression array analysis of NUP98-HBO1-transduced human cord blood (CB) CD34+ cells. FACS sorted CB CD34+ cells transduced with NUP98-HBO1 fusion or empty vector were subjected to RNA extraction and hybridization on the CodeLink Human Whole Genome Bioarray.