scRNA-seq of pancreatic islets from male and female aged mice with Fltp-reporter sorting

Single-cell RNA sequencing of pancreatic islets from aged male and female mice. To disect islet heterogeneity we included both sexes and sorted cells based on maturity marker (Fltp). Islets of Langerhans isolated from the Fltp lineage tracing mouse model Fltp iCre mTmG (Lange, et al. 2012; https://doi.org/10.1016/j.diff.2011.11.003) in male and female mice older than 2 years. We sorted Fltp negative (tomato positive), Fltp lineage positive (GFP positive), and Fltp transient (double positive) populations and generated separate libraries for each population. Male and female mice were pulled together after sorting.