Transcriptome sequencing of HUVECs

We analyzed the global effect of SOX9 overexpression by sequencing the transcriptomes of HUVECs transduced with SOX9 expression plasmid or an empty vector control. Cells were transduced with plasmids and 4 days after total RNA was extracted. Three individual experiments were performed. Sample quality control was performed using the Agilent 2100 Bioanalyzer. Qualifying samples were then prepped following the standard protocol for the NEBnext Ultra ii Stranded mRNA (New England Biolabs). Sequencing was performed on the Illumina NextSeq 500 with Paired End 42bp × 42bp reads