Gene Expression Profile in Caco-2 Cells Treated With 13-HPODE.. Homo sapiens

Lipid peroxides (LOOH) have been implicated in the pathology of diverse diseases, such as gut disease, cardiovascular disease, and cancer. Peroxidized lipids abound in processed food. Recently, RNA Sequencing (RNA-seq) has been widely used to profile gene expression. To characterize gene expression and pathway dysregulation upon exposure to peroxidized linoleic acid, we incubated human intestinal epithelial cells (Caco-2) with 100uM of 13-hydroperoxyoctadecadienoic acid (13-HPODE), linoleic acid (LA) or hydrogen peroxide (H2O2) for 24h. Total RNA was extracted for library preparation and Illumina HiSeq sequencing. This study provides insights into mechanisms by which 13-HPODE alters cellular and metabolic processes, possible involvement in mitochondrial dysfunction-related disorders and proposes potential therapeutic strategies to treat LOOH-related pathologies.