Transcriptome comparison for wheat stripe rust fungus during the infeciton of wheat and barberry

To clarify the different mechanisms deployed by wheat stripe rust fungus (Puccinia striiformis f. sp. tritici, Pst) urediospores and basidiosopres during the infection of wheat and barberry, transcriptomes of both interactions were compared. Two Pst isolates, CYR32 and V26, were inoculated on wheat and barberry leaves. Then wheat leaf samples at 2 dpi and barberry leaf samples at 4 dpi were collected. Since the fungal RNA at the early stage of infection represents only a small fraction of the total RNA from infected leaves, we constructed normalized cDNA library to increase the proportion of pathogen RNA that enabled us to detect expressed fungal genes more readily. Data from normalized library were used for qualitative comparison of expressed genes of two Pst isolates in wheat and barberry. Meanwhile, barberry leaf samples from 3 and 4 dpi as well as wheat leaf samples from 1 and 2 dpi were collected, and non-normalized libraries were constructed for quantitative analysis of transcriptional levels for Pst genes during the early stage of infection of two hosts.