Enzyme Responsive Janus Nanoprobe for Photoacoustic Imaging-guided Enhanced Radiosensitization and Chemodynamic Therapy of Tumor

we developed a biomarker responsive AuNR-based Janus nanoprobe (AMCGL) by integrating MnO2 on one end of AuNRs and specific ligands on the other end. In the tumor microenvironment, highly expressed transglutaminase (TGase) facilitates the formation of isopeptide linkages between glutamine (Gln) and lysine (Lys), which in turn induces the aggregation of AuNRs. Meanwhile, the highly expressed glutathione (GSH) degrades MnO2 to trigger chemodynamic therapy. This in-situ aggregation approach amplifies the intensity of PA imaging, extends the time windows of PA imaging, and enhances the radiosensitization effect. The integrated strategy paves the way for AuNRs with specific responsive assembly and functional activation at the tumor site, facilitating PA imaging-guided enhanced radiosensitization and chemodynamic therapy of tumor.