ATAC seq of isolated primary preadipocytes from male and female mouse gWAT and iWAT on multiple days of differentiation

A map of open chromatin in primary preadipocytes isolated from male and female mouse gWAT and iWAT on days -1, 0, 4h, 1, 2, 7 and mature stage of adipogenic differentiation. On indicated days, cells were permeabilized and nuclei released. Tagmentation was performed according to Buenrostro et al. 2015. Tagmented DNA was amplified by PCR and the libraries assessed on a Bioanalyzer, followed by 75 bp paired end sequencing on an Illumina HiSeq4000 platform.