INTS6 controls DNA:RNA hybrids autoregulation at double-strand break through Senataxin

The fidelity of transcription is essential for genome stability, especially when DNA damage happens. However, how the transcription cycle is regulated remains unknown. Here, we found that INTS6 is a fundamental player, which recruits PP2A to double-strand breaks (DSBs) and functions together with senataxin (SETX). The in-depth insight of ChromeRNA-seq shows that INTS6 is not only involved in global transcription termination but also the de novo transcription termination. Therefore, INTS6 is a great target for future translational studies. Overall design: To investigate the importance of INTS6 in regulating transcription at genome-wide level, we emplyed the chromatin associated RNA sequencing to detect the nascent transcripts around DSBs in both mock and INTS6-depleted cells.