Genomewide profiling of differentially H3K4me3 marked genes in colon cancer cells versus normal colon epithelial cells.

To identify differentially marked genes with a narrow or broad H3K4me3 pattern, we performed ChIP-seq using formaldehyde crosslinked chromatin from three colorectal cancer cell lines and normal colonic epithelial cell line and was digested with micrococcal nuclease to the extent that 70% of the chromatin was cleaved to mononucleosomes. To explore the differentially marked genes with a narrow or broad H3K4me3 pattern, we performed ChIP-seq using formaldehyde crosslinked three colorectal cancer cell lines and normal colonic epithelial cells.