RNA Pol II ChIP-seq analysis after THZ1 treatment in TL-Om1 cells
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https://www.ncbi.nlm.nih.gov/sra/SRP082213
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资源简介：
ChIP-seq analysis was performed in an adult T-cell leukemia/lymphoma cell line (TL-Om1) to analyze DNA bindings of RNA polymerase II (Pol II) after treatment with the THZ1 CDK7 inhibitor. Overall design: TL-Om1 cells were treated with control DMSO or THZ1 for 3 hours. The cells were crosslinked with formaldehyde for 10 min. DNA was enriched by chromatin-immunoprecipitation (ChIP) and analyzed by Illumina Hiseq 4000. A sample of whole cell extract (WCE) was sequenced and used as the background to determine enrichment. ChIP was performed using an antibody against RNA polymerase II (Abcam, ab817: 8WG16).
创建时间：
2018-01-05
